To be correct for bridging strength between front and rear axles I would have the 50x50 at the top of the frame and 25x25 underneath in tension.
Don't suppose that would be so good for the dynamic loads
I could do a 50x50 ladder chassis and then use 25x25 over the top to create a space frame with lots of diagonal bracing. With only three wheels I am not sure that there will be much in the way of chassis twist from end to end. It will still need to be stiff front to back though as there is 110kg motor, 160kg batteries and 150kg of driver and passenger.
It will be sort of sevenesque in proportion with the main complication of the big motor canterlevered off the back of the cockpit and the swing arm pivoting from the same place.
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